Detection of avian influenza virus of H9 subtype in the feces of experimentally infected chickens by RT–PCR
نویسندگان
چکیده مقاله:
Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, AI viruses are detected most frequently between days 3 and 7 post infection (p.i.). The RT-PCR assay was able to detect, at least, 103.5 EID50 of AI viruses in the allantoic fluid. The RT-PCR assay did not show any cross-reactivity with some other avian respiratory viruses. In comparison with virus isolation (VI) assay, the relative sensitivity, specificity, correlation rate and positive predictive value of the RT-PCR were 80%, 84%, 82% and 83%, respectively. The κ index of agreement between the two tests were substantial (κ = 0.64). The results proved that the RT-PCR assay could be a reliable and rapid alternative to VI assay for detection of AI viruses A H9 subtype H9 in fecal specimens.
منابع مشابه
detection of avian influenza virus of h9 subtype in the feces of experimentally infected chickens by rt–pcr
avian influenza (ai) is a viral respiratory disease of domestic and wild birds. in the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. cloacal swabs collected from chickens experimentally infected with h9 subtype ai virus, used in a reverse transcription-polymerase chain reaction (rt-pcr) assay for detection of ai. in infected animals, ai...
متن کاملEvaluation of H9N2 avian influenza virus dissemination in various organs of experimentally infected broiler chickens using RT-PCR
Widespread occurrence of H9N2 low pathogenic avian influenza (LPAI) viruses in many Asian countriesduring the past decade has resulted in the need for evaluation of the pathogenesis of H9N2 virus infection. In this study, tissue tropism and dissemination of A/Chicken/Iran/772/1998(H9N2) virus throughout the body of broiler chickens were investigated. The clinical signs, gross lesions and antibo...
متن کاملstudy of cohesive devices in the textbook of english for the students of apsychology by rastegarpour
this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...
Optimizing the process of inactivating influenza virus subtype H9N2 by formalin in the production of killed avian influenza vaccine
Avian influenza is one of the most important diseases in avian industry, which also threats human population. Thus, vaccination is necessary for controlling this viral disease. In this study, killed vaccine of avian influenza subtype H9N2 and formalin solution (for virus inactivation) were used. It is necessary to study the effect of different factors such as formalin concentration, as well as ...
متن کاملApplying conserved peptides of NS1 Protein of avian influenza virus to differentiate infected from vaccinated chickens
Avian influenza (AI) is a highly contagious disease in poultry and outbreaks can have dramatic economic and health implications. For effective disease surveillance, rapid and sensitive assays are needed to detect antibodies against AI virus (AIV) proteins. In order to support eradication efforts of avian influenza (AI) infections in poultry, the implementation of “DIVA” vaccination strategies, ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 62 شماره 4
صفحات 181- 189
تاریخ انتشار 2007-12-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023